IL-7 ELISA Kit

Produktnummer ABIN5026943

Dieses Human IL-7 ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human IL-7 zu quantifizieren.

Kurzübersicht für IL-7 ELISA Kit (ABIN5026943)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8-500 pg/mL

Applikation: ELISA

Proben: Plasma, Serum

Produktdetails IL-7 ELISA Kit

Untere Nachweisgrenze: 7.8 pg/mL

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of Human IL-7 concentrations in serum, plasma and other biological fluids.

Analytische Methode: Quantitative

Sensitivität: < 4.69 pg/mL

Bestandteile: plate, standard, diluent, antibodies

Benötigtes Material: pipettes, tubes, reader, incubator

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This assay employs an antibody specific for Anti-humanIL-7 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-7 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-humanIL-7antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-7 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: protect your eyes

Lagerung: 4 °C

Details zu IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Andere Bezeichnung: IL-7 (Interleukin 7)

Pathways: JAK-STAT Signalweg